The Han lab features a fully automated custom spinning disk/TIRF system, located in HSTEM 357A, that is managed and shared with other users at Michigan Tech. This confocal/TIRF imaging system utilizes Nikon optics and offers four selectable laser wavelengths through an AOTF (402, 488, 561, and 647). The spinning disk arm includes a Yokogawa CSU-X1 spinning disc confocal head modified with Borealis technology for flat illumination and enhanced light collection efficiency. Reflected light from the SD Confocal is captured by the Prime BSI sCMOS back-illuminated camera. The TIRF arm employs the four laser sources by automatically directing the optical fiber of the laser to the TIRF illumination system. Reflected light from the TIRF system is also captured by the same ORCA-Flash sCMOS camera, utilizing a manual brightfield bypass module designed for Yokogawa. This microscope is further equipped with the pE-800 solid-state illuminator, featuring a standard 4-wavelength filter cube set (402, 488, 561, and 647) and a FRET-specific setup for the CFP/YFP FRET pair. The entire system is housed within an Okolab stage-top incubation unit, which provides temperature and CO2 control for imaging mammalian cells. The system operates on MicroManager software.

The Han lab has the optical tweezers housed in the same room with the SD confocal microscope. It is Thorlabs' OTKB(/M) system, a complete optical trapping setup designed for biological research. This laser-based system utilizes an inverted microscope for compatibility with typical cell samples. The system has a camera for video recording and can be adapted for fluorescence and other microscopy techniques.